Cell

Preparation: unfixed tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: spinning disk confocal microscopy Parameter imaged: fluorescence microscopy Source of contrast: distribution of a specific protein Visualization methods: EGFP Processing history: unprocessed raw data

Preparation: unfixed tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: spinning disk confocal microscopy Parameter imaged: fluorescence microscopy Source of contrast: distribution of a specific protein Visualization methods: EGFP Processing history: unprocessed raw data

Preparation: unfixed tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: spinning disk confocal microscopy Parameter imaged: fluorescence microscopy Source of contrast: distribution of a specific protein Visualization methods: EGFP Processing history: unprocessed raw data

Originally a reproduction of the EFO/Cellosaurus/DepMap/CCLE scenario posed in the Biomappings paper, this configuration imports several different cell and cell line resources and identifies mappings between them.

Preparation: unfixed tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: spinning disk confocal microscopy Parameter imaged: fluorescence microscopy Source of contrast: distribution of a specific protein Visualization methods: EGFP Processing history: unprocessed raw data

We thank P. Golstein, R. Horvitz, A. Mudge, and R. Parnaik for helpful comments on the manuscript; J. Scholes for providing the drawings for Figure 2Figure 2; and J.-C. Ameisen, J. Burne, P. Golstein, R. Horvitz, K. Plasleitt, K. Roberts, and G. Stanfield for providing electron micrographs. Because of the limited number of references allowed, we were unable to cite many important papers; we apologize to their authors.

Epithelial cells form energetically costly cell-cell adhesions in response to mechanical forces. How cells obtain their energy during this event is unclear. Activity of a key regulator of cell metabolism, the AMP-activated protein kinase (AMPK), is now shown to be mechanoresponsive, and thus can bridge adhesion mechanotransduction and energy homeostasis.