Sialyl Transferase-EGFP transfected HeLa cells demonstrating the redistribution of the Golgi Apparatus to the Endoplasmic Reticulum upon treatment with Brefeldin A (BFA). Images were collected as a 4D time series (z-stack over time). z-stacks of 48 individual timepoints are included in this collection of files as well as the movie SiEGFP_BFA.avi, which is the maximum projection of the z-stacks at the individual time points. BFA was added according to the following protocol: Timepoint1: Before
doi.orgSialyl Transferase-EGFP transfected HeLa cells demonstrating the redistribution of the Golgi Apparatus to the Endoplasmic Reticulum upon treatment with Brefeldin A (BFA). Images were collected as a 4D time series (z-stack over time). z-stacks of 48 individual timepoints are included in this collection of files as well as the movie SiEGFP_BFA.avi, which is the maximum projection of the z-stacks at the individual time points. BFA was added according to the following protocol: Timepoint1: Before
doi.orgSialyl Transferase-EGFP transfected HeLa cells demonstrating the redistribution of the Golgi Apparatus to the Endoplasmic Reticulum upon treatment with Brefeldin A (BFA). Images were collected as a 4D time series (z-stack over time). z-stacks of 48 individual timepoints are included in this collection of files as well as the movie SiEGFP_BFA.avi, which is the maximum projection of the z-stacks at the individual time points. BFA was added according to the following protocol: Timepoint1: Before
doi.orgRecently, cell signaling has been shown to be required for the formation of germ cells in the mouse embryo, direct observation of germ cell migration in living mouse embryos has been achieved, novel genes that control germ-cell migration have been identified in Drosophila, and the roles of many components of germ plasm in several species have become clearer.
dx.doi.orgSialyl Transferase-EGFP transfected HeLa cells demonstrating the redistribution of the Golgi Apparatus to the Endoplasmic Reticulum upon treatment with Brefeldin A (BFA). Images were collected as a 4D time series (z-stack over time). z-stacks of 48 individual timepoints are included in this collection of files as well as the movie SiEGFP_BFA.avi, which is the maximum projection of the z-stacks at the individual time points. BFA was added according to the following protocol: Timepoint1: Before
doi.orgHela cells transiently transfected with EGFP-GL-GPI show the organization of the membrane thru the distribution of GPI anchored protein. This image is the maximum z projection image that accompanies the related z stack. The images were collected on a Zeiss spinning disk confocal microscope using a 63 X 1.4 NA oil immersion objective. 56 z slices at 0.27 micron intervals were collected with a 450 ms exposure using a 488 ht.
doi.orgSialyl Transferase-EGFP transfected HeLa cells demonstrating the redistribution of the Golgi Apparatus to the Endoplasmic Reticulum upon treatment with Brefeldin A (BFA). Images were collected as a 4D time series (z-stack over time). z-stacks of 48 individual timepoints are included in this collection of files as well as the movie SiEGFP_BFA.avi, which is the maximum projection of the z-stacks at the individual time points. BFA was added according to the following protocol: Timepoint1: Before
doi.orgThis is the raw fastq files, processed count table, peptide mapping files, raw flow cytometry files, and single cell sequence Seurat object for the manuscript entitled Detection of Cell-Cell Interactions via Photocatalytic Cell Tagging. The file "File_Manifest.csv" contains a description of each file and to what it pertains. Raw sequencing files for the single cell sequencing may be found in the following GEO entry GSE206140 (link https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE206140).
doi.orgSialyl Transferase-EGFP transfected HeLa cells demonstrating the redistribution of the Golgi Apparatus to the Endoplasmic Reticulum upon treatment with Brefeldin A (BFA). Images were collected as a 4D time series (z-stack over time). z-stacks of 48 individual timepoints are included in this collection of files as well as the movie SiEGFP_BFA.avi, which is the maximum projection of the z-stacks at the individual time points. BFA was added according to the following protocol: Timepoint1: Before
doi.org